Reactions of bone, liver and kidney tissues to orthopaedic implants with silver nanoparticle doped hydroxyapatite coatings: microscopic examination in a rabbit model

Łukasz Łapaj, Waldemar Woźniak, Paweł Chodór, Jan Zabrzyński, Łukasz Paczesny

Abstract


Background: Periprosthetic joint infections are severe complications of arthroplasty, difficult to manage due to biofilm formation on the components. Recently, the use of silver nanoparticles (SNs) has emerged as a method of preventing biofilm formation on orthopaedic implants, however little is known about the systematic toxicity of SNs.

Aim: This study used a rabbit model to examine the tissue response of bone, liver and kidney to prototype components with SN doped hydroxyapatite (HA) coatings.

Materials and methods: Twelve prototype implants (six with HA, six with SN doped HA coatings) were implanted into the femora of twelve New Zealand Rabbits. After 6 weeks, the animals were euthanised, their femora and samples of livers and kidneys harvested to prepare microscopic slides. The slides were examined for the presence inflammatory or toxic reactions to SNs, implants were examined using scanning electron microscopy (SEM) to determine structural changes related to implantation and verify retention of SNs in vivo.

Results: SEM demonstrated that SNs formed submicron conglomerates, which were retained after 6 weeks in vivo and did not interfere with osseointegration. Histologic studies of bone fragments demonstrated no signs of acute toxicity and inflammation. No inflammatory reaction was observed in kidneys, although in some samples signs of acute renal failure related to euthanasia were found. No severe toxic reaction was found in liver samples, however fatty degeneration of liver was found in some animals.

Conclusions: This study documented good osteointegration of implants with SN doped HA coatings with low systematic toxicity of SNs.


Keywords


osseointegration; silver nanoparticles; hydroxyapatite coatings

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References


Vanhegan I, Malik A, Jayakumar P, Islam SU, Haddad F. A financial analysis of revision hip arthroplasty: the economic burden in relation to the national tariff. J Bone Joint Surg Br. British Editorial Society of Bone and Joint Surgery; 2012;94(5):619–23.

Kose N, Otuzbir A, Pekşen C, Kiremitçi A, Doğan A. A silver ion-doped calcium phosphate-based ceramic nanopowder-coated prosthesis increased infection resistance. Clin Orthop Relat Res. 2013;471(8):2532–9.

Besinis A, Hadi SD, Le H, Tredwin C, Handy R. Antibacterial activity and biofilm inhibition by surface modified titanium alloy medical implants following application of silver, titanium dioxide and hydroxyapatite nanocoatings. Nanotoxicology; 2017;11(3):327–38.

Cheng Y, Gao B, Liu X, Zhao X, Sun W, Ren H, Wu J. In vivo evaluation of an antibacterial coating containing halogenated furanone compound-loaded poly (l-lactic acid) nanoparticles on microarc-oxidized titanium implants. International journal of nanomedicine.; 2016;11:1337.

Kleiner DE, Brunt EM, Van Natta M, Behling C, Contos MJ, Cummings OW et al. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology. 2005;41(6):1313–21.

Lucaciu O, Băciuţ M, Băciuţ G, Câmpian R, Soriţău O, Bran S, et al. Tissue engineered bone versus alloplastic commercial biomaterials in craniofacial reconstruction. Rom J Morphol Embryol. 2010;51(1):129–36.

Ciobanu CS, Iconaru SL, Chifiriuc MC, Costescu A, Le Coustumer P, Predoi D. Synthesis and antimicrobial activity of silver-doped hydroxyapatite nanoparticles. BioMed Research International; 2013;2013.

Kim T-H, Kim M, Park H-S, Shin US, Gong M-S, Kim H-W. Size-dependent cellular toxicity of silver nanoparticles. J Biomed Mater Res A. 2012;100(4):1033–43.

Xie C-M, Lu X, Wang K-F, Meng F-Z, Jiang O, Zhang H-P et al. Silver nanoparticles and growth factors incorporated hydroxyapatite coatings on metallic implant surfaces for enhancement of osteoinductivity and antibacterial properties. ACS applied materials & interfaces; 2014;6(11):8580–9.

Punke C, Zehlicke T, Just T, Holzhüter G, Gerber T, Pau HW. Matrix change of bone grafting substitute after implantation into guinea pig bulla. Folia Morphol (Warsz). 2012;71(2):109–14.

Kose N, Çaylak R, Pekcsen C, Kiremitçi A, Burukoglu D, Koparal S et al. Silver ion doped ceramic nano-powder coated nails prevent infection in open fractures: in vivo study. Injury; 2016;47(2):320–4.




DOI: http://dx.doi.org/10.20883/jms.2018.291

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